E. coli T7 S30 Extract System for Circular DNA from Promega

Product Specs
ItemE. coli T7 S30 Extract System for Circular DNA
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberL1130
Quantity30 reactions

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Genome-Wide Transcription Factor DNA Binding Sites and Gene Regulatory Networks in Clostridium thermocellumFrontiers in MicrobiologySeptember 7, 2021Skyler D. Hebdon, Alida T. Gerritsen, Yi-Pei Chen, Joan G. Marcano, Katherine J. Chouthermophilic and anaerobic bacterium. The E. coli T7 S30 Extract System for Circular DNA (Promega, cat. no L1130) was used for IVTT of putative TFs according to the manufacturer’s recommendation. For a large-scale

(2) BioBits™ Bright: A fluorescent synthetic biology education kitScience AdvancesAugust 3, 2018Jessica C. Stark et al.FD-CF reactions, similar cell-free systems are available commercially from companies such as Promega (L1130). Lyophilization of cell-free reactions FD-CF reactions were prepared according to the recipe above,

(3) Mechanistic and structural basis of bioengineered bovine Cathelicidin-5 with optimized therapeutic activityScientific ReportsApril 12, 2017Bikash R. Sahoo, Kenta Maruyama, Jyotheeswara R. Edula, Takahiro Tougan, Yuxi Lin, Young-Ho Lee, Toshihiro Horii, Toshimichi FujiwaraLuciferase T7 Control DNA plasmid (L482B, Promega) was examined using E. coli T7 S30 Extract System (L1130, Promega). The complete protocol for reaction mixture and final solution preparation was followed as

(4) Crystal structure of an invertebrate cytolysin pore reveals unique properties and mechanism of assemblyNature CommunicationsSeptember 1, 2016Marjetka Podobnik et al.in vitro transcription and translation by using an E. coli T7-S30 extract system for circular DNA (Promega, no. L1130). As per the manufacturer's instructions, reaction mixture was supplemented with L-[35S]methionine (Perkin Elmer

(5) Cell-free protein expression systems in microdroplets: Stabilization of interdroplet bilayersBiomicrofluidicsJanuary 1, 2013Mark S. Friddin, Hywel Morgan, Maurits R. R. de Planqueexperiments, i.e., at least at a daily basis. D. Cell-free expression mixtures and reference samples The L1130 E. coli T7 Extract System for Circular DNA (Promega, WI, USA), the EasyXpress Protein Synthesis Kit

(6) Tetrameric assembly of KvLm K ⁺ channels with defined numbers of voltage sensorsProceedings of the National Academy of SciencesOctober 16, 2012Ruhma Syeda, Jose S. Santos, Mauricio Montal, Hagan Bayleytetramers. The IVTT kit contained an E. coli T7-S30 extract optimized for circular DNA (Promega no. L1130). The complete amino acid mixture (5 μL, 1 mM) and premix solution (20 μL) were mixed with L-½ 35 Smethionine

(7) Tuning the tetraethylammonium sensitivity of potassium channel Kcv by subunit combinationJournal of General PhysiologyApril 1, 2012Qiulin Tan, Brandon Ritzo, Kai Tian, Li-Qun Guin vitro transcription and translation using the kit Escherichia coli T7 S30 Extract System for Circular DNA (L1130; Promega). To produce heterotetramers, 4 µl of mixed plasmid of tagged Kcv DNA and untagged Kcv DNA

(8) Identifying Assembly-Inhibiting and Assembly-Tolerant Sites in the SbsB S-Layer Protein from Geobacillus stearothermophilusJournal of Molecular BiologyOctober 8, 2009Helen Kinns, Helga Badelt-Lichtblau, Eva Maria Egelseer, Uwe B. Sleytr, Stefan Howorka

(9) The Surface Location of Individual Residues in a Bacterial S-Layer ProteinJournal of Molecular BiologyJanuary 8, 2008Helen Kinns, Stefan Howorka

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